Modeling long-term diabetes and related complications in rats J Pharmacol Toxicol Methods. Mar-Apr ; Epub Nov Gyömrői út Electronic address: erika.
DOI: The aims of this study were to establish a model of long-term diabetes with sustained medium scale hyperglycemia and characterize the diabetic nephropathy treatment changes detectable after 4months, with particular respect to dependence on the degree of hyperglycemia. Methods: Streptozotocin-induced diabetic CFY rats were subjected to four different insulin substitution protocols to achieve different levels of glycemic control Diabetic groups.
Eyes were investigated by ophthalmoscopy, kidney function by urine analysis, and neuropathy by functional tests. Retinal and renal morphological evaluations were performed by histology, immuno-histochemistry and electron microscopy.
- A diabéteszes nefropátia a felnőttkori veselégtelenség vezető oka, a megnövekedett kardiovaszkuláris CV kockázat fokozott mortalitáshoz vezet.
- Diabetes insipidus renalis diagnostik
- Endokrinológia a cukorbetegség kezelésére
Discussion: We established a treatment protocol in rats enabling complex investigation of diabetic retinopathy, nephropathy and neuropathy on a long-term period.
Clearly hyperglycemic dependent parameters of these complications serve as good outcome measures for preclinical trials.
Our results provide a useful basis for designing studies for testing preventative treatments as well as other translational medical research in this field.
Keywords: Anterior segment neovascularization; Chronic model; Cold allodynia; Diabetic nephropathy; Diabetic neuropathy; Diabetic retinopathy; Drug development; Diabetic nephropathy treatment implant; Preclinical model; Streptozotocin.
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